rneasy microarray tissue mini kit #73304  (Qiagen)

Journal: BMC Biology

Article Title: TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity

doi: 10.1186/s12915-020-00816-1

Figure Lengend Snippet: RTEs are upregulated in TBPH mutants. a Microarray results showing upregulated TEs in TBPH-null mutants: the fold changes are reported for both tbph mutant alleles (Δ23 and Δ142) and referred to w 1118 ; TE family and class were also indicated. n = 3 (biological replicates). b Real-time quantitative PCR reveals accord and gypsy transcript levels normalized on Rpl11 (housekeeping) in w 1118 - tbph Δ23 ,elav-GAL4/tbph Δ23 ; UAS-GFP/+ and tbph Δ23 , elav -GAL4/tbph Δ23 ,UAS-TBPH. n = 3 (biological replicates, with 3 technical replicates for each), error bars SEM. c Western blot analysis of Drosophila env levels in Δtb-gypsy-IR (tbph Δ23 , elav -GAL4/tbph Δ23 ; UAS-gypsy-IR/+), ctrl (tbph Δ23 , elav -GAL4/+), Δtb-GFP-IR (tbph Δ23 , elav -GAL4/tbph Δ23 ; UAS-GFP-IR/+), and Δtb-TBPH (tbph Δ23 , elav -GAL4/tbph Δ23 ,UAS-TBPH). Adult brains, 1 day old, were probed with anti-ENV and alpha-tubulin antibodies. The same membrane was probed with the two antibodies and the bands of interest were cropped. Quantification of normalized amounts and SD was reported below each lane. n = 2 (biological replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 calculated by one-way ANOVA, error bars SEM. Individual data values are provided in Additional file : Individual Data Values.pdf

Article Snippet: RNA, both from adult dissected brains and adult heads, was extracted with the RNeasy Microarray tissue kit (QIAGEN #73304).

Techniques: Microarray, Mutagenesis, Real-time Polymerase Chain Reaction, Western Blot, Membrane